Associate Professor

　　　　　Hiroyuki Morimoto〔DDS, Ph.D.〕

Key Words

Double-stranded RNA dependent protein kinase (PKR), Apoptosis, Protein ineraction

Publications

1. Haneji T, Morimoto H, Morimoto Y, Shirakawa S, Kobayashi S, Kaneda C, Shima H and Nagao M: Subcellular localization of protein phosphatase type 1 isotypes in mouse osteoblastic cells. Biochemical and Biophysical Research Communications 248, 39-43, 1998.

2. Morimoto Y, Morimoto H, Kobayashi S, Ohba T and Haneji T: Extracts of Actinobacillus actinomycetemcomitans induce apoptotic cell death in human osteoblastic MG63 cells. Journal of Dental Research 78, 735-742, 1999.

3. Morimoto Y, Morimoto H, Kobayashi S, Ohba T, Haneji T: The protein phosphatase inhibitors, okadaic acid and calyculin A, induce apoptosis in human submandibular gland cell line HSG cells. Oral Diseases, 5, 104-110, 1999.

4. Nakamura K, Morimoto Y, Morimoto H, Kobayashi S, Kido H, Morikawa M, Haneji T: Prilocaine induces apoptosis in osteoblastic cells. Canadian Journal of Anaesthesia, 46, 476-482, 1999.

5. Morimoto H, Morimoto Y, Kido H, Kobayashi S, Ohba T and Haneji T: Inhibitors of protein synthesis and RNA synthesis protect against okadaic acid-induced apoptosis in human osteosarcoma cell line MG63 cells but not in Saos-2 cells. Journal of Bone and Mineral Metabolism 17 (4), 266-273, 1999.

6. Morimoto Y, Morimoto H, Okamura H, Nomiyama K, Nakamuta N, Kobayashi S, Kito S, Ohba T, Haneji T: Upregulation of the expression of Fas antigen and Fas ligand in a human submandibular gland ductal cell line by okadaic acid. Archives of Oral Biology, 45 (8), 657-666, 2000.

7. Morimoto Y, Kito S, Ohba T, Morimoto H, Okamura H, Haneji T: Alteration of argyrophilic nucleolar organizer region associated (Ag-NOR) proteins in apoptosis-induced human salivary gland cells and human squamous carcinoma cells. Journal of Oral Pathology and Medicine, 30 (4), 193-199, 2001.

8. Okamura H, Morimoto H, Haneji T: Peplomycin-induced apoptosis in oral squamous carcinoma cells depends on bleomycin sensitivity. Oral Oncology, 37 (4), 379-385, 2001.

9. Morimoto H, Okamura H and Haneji T: Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells. The Journal of Histochemistry and Cytochemistry 50 (9), 1187-1193, 2002.

10. Okamura H, Morimoto H, Fujita M, Nasu F, Sasaki E, Haneji T: Suppression of Egr-1 expression in human squamous carcinoma cells by okadaic acid. Oral Oncology, 38 (8), 779-784, 2002.

11. Kito S, Shimizu K, Okamura H, Yoshida K, Fujita M, Morimoto H, Morimoto Y, Ohba T, Haneji T: Cleavage of nucleolin and argyrophilic nucleolar organizer region associated proteins in apoptosis-induced cells. Biochemical and Biophysical Research Communications 300 (4), 950-956, 2003.

12. Yoshida K, Okamura H, Morimoto H, Nagata T, Haneji T: Okadaic acid stimulates the receptor activator of nuclear factor-kappa B ligand (RANKL) in mouse osteoblastic cells. Biomedical Research, 14 (2), 126-132, 2003.

13. Sasaki E, Okamoto Y, Yoshida K, Okamura H, Shimizu K, Nasu F, Morimoto H, Haneji T: Transblot and cytochemical identification of avidin-interacting proteins in mitochondria of cultured cells. Histochemistry and Cell Biology, 120 (4), 327-333, 2003.

14. Yamashita K, Dennid, JE, Lennon DP, Morimoto H, Kitamura S, Caplan A: Dental pulp cells with multi-potential for differentiation to odontoblast and chondroblast. Journal of Hard Tissue Biology 12, 49-55, 2003.

15. Yamashita K, Morimoto H, Sanjabad AS, Raji AR, Fereydoon SA, Yamauchi R, Maeda E, Kitamura S: Morphological features of the osteopetrotic Op/Op mouse skeleton. Dentistry in Japan, 39, 19-22, 2003.

16. Morimoto H: Type 1 protein phosphatase controls biological processes in cultured human osteoblastic cells. Journal of Oral Biosciences, 46 (4), 270-277, 2004.

17. Fujita M, Goto K, Yoshida K, Okamura H, Morimoto H, Kito S, Fukuda J, Haneji T: Okadaic acid stimulates expression of Fas receptor and Fas ligand by activation of nuclear factor kappa-B in human oral squamous carcinoma cells. Oral Oncology, 40 (2), 199-206, 2004.

18. Yamashita K, Eguchi S, Morimoto H, Hanawa T, Ichikawa T, Nakajo N, Kitamura S: Extracellular matrix formed by MC3T3-E1 osteoblast-like cells cultured on titanium. 1. Anchor structure. Connective Tissue 36, 1-8, 2004.

19. Eguchi S, Yamashita K, Morimoto H, Ichikawa T, Nakajo N, Kitamura S: Extracellular matrix formed by MC3T3-E1 osteoblast-like cells cultured on titanium. 2. Collagen fiber formation. Connective Tissue 36, 9-15, 2004.

20. Okamura H, Yoshida K, Morimoto H, Sasaki E, Haneji T: Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells. International Journal of Oncology, 25 (4), 1031-1037, 2004.

21. Morimoto H: Type 1 protein phosphatase controls biological processes in cultured human osteoblastic cells. Journal of Oral Biosciences 46, 270-277, 2004.

22. Morimoto H, Okamura H, Yoshida K, Kitamura S and Haneji T: Double-stranded RNA mediates selective gene silencing of protein phosphatase type 1 delta isoform in HEK-293 cells. Journal of Enzyme Inhibition & Medical Chemistry 19, 327-331, 2004.

23. Morimoto H, Okamura H, Yoshida K, Kitamura S and Haneji T: Okadaic acid induces apoptosis through double-stranded RNA-dependent protein kinase/eukaryotic initiation factor-2α pathway in human osteoblastic MG63 cells. Journal of Biochemistry 136, 433-438, 2004.

24. Okamura H, Yoshida K, Morimoto H, Haneji T: PTEN expression by Egr-1 transcription factor in calyculin A-induced apoptotic cells. Journal of Cellular Biochemistry 94 (1), 117-125, 2005.

25. Morimoto H, Ozaki A, Okamura H, Yoshida K, Kitamura S and Haneji T: Okadaic acid induces tyrosine phosphorylation of IκBα that mediated by PKR pathway in human osteoblastic MG63 cells. Molecullar and Cellular Biochemistry 276, 211-217, 2005.

27. Yoshida K, Okamura H, Amorim BR, Ozaki A, Tanaka H, Morimoto H and Haneji T: Double-stranded RNA-dependent protein kinase is required for bone calcification in MC3T3-E1 cells in vitro. Experimental Cell Research 311, 117-125, 2005.

28. Ozaki A, Morimoto H, Tanaka H, Okamura H, Yoshida K, Amorim BR, Haneji T: Okadaic acid induces phosphorylation of p65NF-?B on serine 536 and activates NF-κB transcriptional activity in human osteoblastic MG63 cells. Journal of Cellular Biochemistry, 99 (5), 1275-1284, 2006.

29. Morimoto H, Ozaki A, Okamura H, Yoshida K, Amorim BR, Tanaka H, Kitamura S, Haneji T: Differential expression of protein phosphatase type 1 isotypes and nucleolin during cell-cycle arrest, Cell Biochemistry and Function, 25 (4), 369-375, 2007.

30. Okamura H, Yoshida K, Sasaki E, Qui LH, Amorim BR, Morimoto H, Haneji T: Expression of PTEN and Akt phosphorylation in Lipopolysaccharide-treated NIH3T3 cells, Cell Biology International, 31 (2), 119-125, 2007.

31. Amorim BR, Okamura H, Yoshida K, Qiu LH, Morimoto H, Haneji T: The transcriptional factor Osterix directly interacts with RNA helicase A. Biochemical and Biophysical Research Communications, 355 (2), 347-351, 2007.

32. Tanaka H, Yoshida K, Okamura H, Morimoto H, Nagata T, Haneji T: Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-κB in human osteoblastic osteosarcoma MG63 cells. International Journal of Oncology, 31 (2), 389-396, 2007.

33. Akita K, Okamura H, Yoshida K, Morimoto H, Ogawa-Iyehara H, Haneji T: Cobalt chloride induces apoptosis and zinc chloride suppresses cobalt-induced apoptosis by bcl-2 expression in human submandibular gland HSG cells. International Journal of Oncology, 31 (4), 923-929, 2007.

34. Sato N, Morimoto H, Baba R, Nakamata J, Doi Y, Yamaguchi K. Functional expression of double-stranded RNA-dependent protein kinase in rat intestinal epithelial cells. Journal of Cellular Biochemistry, 110 (1), 104-111, 2010.

35. Teramachi J, Morimoto H, Baba R, Doi Y, Hirashima K, Haneji T: Double stranded RNA-dependent protein kinase is involved in osteoclast differentiation of RAW 264.7. Experiment Cell Research, 316 (19), 3254-3262, 2010.

Social Activities

Japanese Association of Anatomists, The Japanese Society of Microscopy, Japanese Society for Clinical Molecular Morphology, Japanese Association for Oral Biology, Kyushu Dental Society, The Japanses Society for Cell Death Research