Publications of our research

Structure Determination of Ether Lipids of Methanogenic and Other Archaea

S1.	Morii, H., Nishihara, M., Ohga, M., & Koga, Y. (1986) A diphytanyl ether analog of phosphatidylserine from a methanogenic bacterium, Methanobrevibacter arboriphilus. Journal of Lipid Research 27, 724-730.
S2.	Nishihara, M., Morii, H. & Koga, Y. (1987) Structure determination of a quartet of novel tetraether lipids from Methanobacterium thermoautotrophicum. Journal of Biochemistry 101, 1007-1015
S3.	Morii, H., Nishihara, M., & Koga, Y. (1988) Composition of polar lipids of Methanobrevibacter arboliphilicus and structure determination of the signature phosphoglycolipid of Methanobacteriaceae. Agricultural and Biological Chemistry 52, 3149-3156
S4.	Nishihara, M., Morii, H., & Koga, Y. (1989) Heptads of polar ether lipids of an archaebacterium, Methanobacterium thermoautotrophicum: structure and biosynthetic relationship. Biochemistry 28 , 95-102.
S5.	Nishihara,M., & Koga, Y. (1991) Hydroxyarchaetidylserine and hydroxyarchaetidyl-myo-inositol in Methanosarcina barkeri: polar lipids with a new ether core portion. Biochimica et Biophysaca Acta 1082, 211-217.
S6.	Nishihara, M., Utagawa, M., Akutsu, H., & Koga, Y. (1992) Archaea contain a novel diether phosphoglycolipid with a polar head group identical to the conserved core of eucaryal glycosyl phsophatidylinositol. Journal of Biological Chemistry 267, 12432-12435.
S7.	Koga, Y., Nishihara, M , Morii, H. , & Akagawa-Matsushita, M. (1993) The ether polar lipids of methanogenic bacteria. Structures, comparative aspects, and biosynthesis. Microbiological Reviews 57, 164-182.
S8.	Nishihara, M., & Koga, Y. (1995) Two new phospholipids, hydroxyarchaetidylglycerol and hydroxyarchaetidylethanolamine , from the archaea Methanosarcina barkeri. Biochimica et Biophysica Acta 1254, 155-160.
S9.	Morii, H., Eguchi, T., Kakinuma, K., Konig, H. & Koga,Y. (1998) A novel ether core lipid with an H-shaped C80-isoprenoid hydrocarbon chain from the hyperthermophilic methanogen Methanothermus fervidus. Biochimica et Biophysica Acta 1390(3), 339-345.
S10.	Morii, H., Yagi, H., Akutsu, H., Nomura, N., Sako, Y. & Koga, Y. (1999) A novel phosphoglycolipid archaetidyl(glucosyl)inositol with two sesterterpanyl chains from the aerobic hyperthermophilic Archaeon Aeropyrum pernix K1.Biochimica et Biophysica Acta 1436, 426-436.
S11.	Nishihara, M. & Koga, Y. (1999) Gas-liquid chromatographic determination of total glycerophosphate in an aqueous solution. Analytical Biochemistry 276, 260-261
S12.	Nishihara, M. & Koga, Y. (2000) Enzymatic determination of sn-glycerol-1-phosphate. Journal of UOEH 22, 13-18
S13.	Nishihara, M., Nagahama, S., Ohga, M. & Koga, Y. (2000) Straight chain fatty alcohols in the hyperthermophilic archaeon Pyrococcus furiosus. Extremophiles 4, 275-277
S14.	Nishihara, M., Morii, H., Matsuno, K., Ohga, M., Stetter, K.O., and Koga, Y. (2002) Structure analysis by reductive cleavage with LiAlH4 of an allyl ether choline-phospholipid, archaetidylcholine, from the hyperthermophilic Methanoarchaeon Methanopyrus kandleri. Archaea 1, 123-131
S15.	Koga, Y., & Morii, H. (2005) Recent advances in structural research on ether lipids from archaea including comparative and physiological aspects. Bioscience Biotechnology and Biochemistry 69, 2019-2034
S16.	Koga, Y., & Morii, H. (2006) Special methods for the analysis of ether lipid structure and metabolism in archaea. Analytical Biochemistry 348, 1-14

Biosynthesis of ether lipids in archaebacteria

B1.	Morii ,H., & Koga, Y. (1993) Tetraether type polar lipids increase after logarithmic growth phase of Methanobacterium thermoautotrophicum in compensation for the decrease of diether lipids. FEMS Microbiology Letters 109, 283-288.
B2.	Morii, H., & Koga, Y. (1994) Asymmetric topology of diether and tetraether type polar lipids in the membrane of Methanobacterium thermoautotrophicum cells. Journal of Biological Chemistry 269, 10492-10497.
B3.	Nishihara, M., & Koga, Y. (1995) sn-Glycerol-1-phosphate dehydrogenase in Methanobacterium thermoautotrophicum: the key enzyme for biosynthesis of the enantiomeric glycerophosphate backbone of ether phospholipids of the archaebacteria. Journal of Biochemistry 117, 933-935.
B4.	Nishihara, M. & Koga, Y. (1997) Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum : characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea. Journal of Biochemistry 122(3), 572-576.
B5.	Nishihara, M., Yamazaki, T., Oshima, T. & Koga, Y. (1999) sn-glycerol-1-phosphate-forming activities in archaea: separation of archaeal phospholipid biosynthesis and glycerol catabolism by glycerophosphate enantiomers. Journal of Bacteriology 181, 1330-1333
B6.	Eguchi, T., Takyo, H., Morita, M., Kakinuma, K. & Koga, Y. (2000) Unusual double-bond migration as plausible key reaction in the biosynthesis of the isoprenoid membrane lipids of methanogenic archaea. Chemical Communications 2000, 1545-1546
B7.	Morii, H., Nishihara, M. & Koga, Y. (2000) CTP: 2,3-di-O-geranylgeranyl-sn- glycero-1-phosphate cytidyltransferase in the methanogenic archaeon Methanothermobacter thermoautotrophicus. Journal of Biological Chemistry 275, 36568-36574
B8.	Daiyasu, H., Hiroike, T., Koga, Y. & Toh, H. (2002) Analysis of membrane stereochemistry with homology modeling of sn-glycerol-1-phosphate dehydrogenase. Protein Engineering 15, 987-995
B9.	Morii, H. & Koga, Y. (2003) CDP-2,3-Di-O-geranylgeranyl-sn-glycerol:L-serine O-archaetidyltransferase (archaetidylserine synthase) in the methanogenic archaeon Methanothermobacter thermautotrophicus. Journal of Bacteriology 185, 1181-1189
B10.	Koga, Y., Sone, N., Noguchi, S. & Morii, H. (2003) Transfer of pro-R hydrogen from NADH to dihydroxyacetonephosphate by sn-glycerol-1-phosphate dehydrogenase from the archaeon Methanothermobacter thermautotrophicus. Bioscience, Biotechnology and Biochemistry 67, 1605-1608
B11.	Daiyasu, H., Kuma, K., Yokoi, T., Morii, H., Koga, Y., & Toh, H. (2005) A study on archaeal enzymes involved in polar lipids synthesis by an approach linking the information about amino acid sequences, genomic contexts and lipid composition. Archaea 1, 399-410
B12.	Koga, Y., Ohga, M., Tsujimura, H., Morii, H., & Kawarabayasi, Y. (2006) Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information of a hyperthermophilic archaeon Sulfolobus tokodaii strain 7. Biosci. Biotechnol. Biochem. 70, 282-285
B13	Koga, Y. & Morii, H. (2007) Biosynthesis of ether-type polar lipids in archaea and evolutionary considerations. Microbiology and Molecular Biology Reviews 71, 97-120
B14	Morii, H., Eguchi, T., & Koga, Y. (2007) In vitro biosynthesis of ether-type glycolipids in the methanoarchaeon Methanothermobacter thermautotrophicus. Journal of Bacteriology in press

Lipids as a Chemotaxonomic Marker

T1.	Koga, Y. , Akagawa-Matsusita, M., Ohga, M., & Nishihara, M. (1993) Taxonomic significance of the distribution of the component parts of polar lipids in methanogens. Systematic and Applied Microbiology 16, 342-351.
T2.	Koga, Y., Morii, H., Akagawa-Matsushita, M., & Ohga, M. (1998) Correlation of polar lipid composition with 16S rRNA phylogeny in methanogens. Further analysis of lipid component parts. Bioscience, Biotechnology, and Biochemistry 62(2), 230-236.
T3.	Whitman, W.B., Boone, D.R., Koga, Y. and Keswani, J. (2001) Taxonomy of Methanogenic Archaea. Bergey's Manual of Systematic Bacteriology 2nd Edition vol.1, The Archaea and the deeply branching and phototrophic bacteria, pp211-213 Springer-Verlag, New York

Differentiation of Archaea and Bcteria

D1.

Koga, Y., Kyuragi, T., Nishihara, M. & Sone, N. (1998) Did archaeal and bacterial cells arise independently from noncellular precursors? A hypothesis stating that the advent of membrane phospholipid with enantiomeric glycerophosphate backbones caused the separation of the two lines of descent. Journal of Molecular Evolution 46, 54-63.

Application of Lipid Analysis to Ecological Studies of Methanogenic Archaea

E1.	Demizu, K., S. Ohtsubo, S. Kohno, I. Miura, M. Nishihara, and Y. Koga. (1992) Quantitative Determination of Methanogenic Cells Based on Analysis of Ether-Linked Glycerolipids by High-Performance Liquid Chromatography. Journal of Fermentation and Bioengineering 73(2),135-139
E2.	Ohtsubo, S., M. Kanno, H. Miyahara, S. Kohno, Y. Koga, and I. Miura. (1993) A sensitive method for quantification of aceticlastic methanogens and estimation of total methanogenic cells in natural environments based on an anlysis of ether-linked glycerolipids. FEMS Microbiology Ecology 12(1), 39-50
E3.	Nishihara, M., M. Akagawa-Matsushita, Y. Togo, and Y. Koga. (1995) Inference of methanogen groups in waste water treatment digestor sludges based on the analysis of ether lipid component parts. Journal of Fermentation and Bioengineering 79, 400-402
E4.	Asakawa, S., M. Akagawa-Matsushita, Y. Koga, and K. Hayano. (1998) Communities of methanogenic bacteria in paddy field soils with long-term application of organic matter. Soil Biology and Biochemistry 30(3), 299-303
E5.	Sunamura, M., Y. Koga, and K. Ohwada. (1999) Biomass measurement of methanogens in the sediments of Tokyo bay using archaeol lipids. Marine Biotechnology 1, 562-568

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[update 4. 6, 2007 edited by Dept.of Chemistry]