1.
ノルアドレナリントランスポーターの機能調節機序の解析
神経末端からシナプス間隙に放出されたアミンを特異的に細胞内に取り込む膜蛋白質であるアミントランスポーター(ドーパミントランスポーター、ノルアドレナリントランスポーター、セロトニントランスポーター)は、神経伝達を終了させる役割を担っており、麻薬や抗うつ薬の標的分子である。ノルアドレナリントランスポーターに関して発現調節やメンブレントラフィック、活性調節等について検討する。
2.
カテコールアミン分泌機序の解析
分泌に関与する受容体やイオンチャネル、細胞内シグナル伝達の機能について検討する。
3.
内分泌かく乱物質、植物エストロゲンの神経系への影響
＜キーワード＞
ノルアドレナリントランスポーター・カテコールアミン・開口放出・内分泌かく乱物質・植物エストロゲン
1.
Liu M, Yanagihara N, Toyohira Y, Tsutsui M, Ueno S, Shinohara Y. (2007) Dual effects of daidzein, a soy isoflavone, on catecholamine synthesis and secretion in cultured bovine adrenal medullary cells. Endocrinorogy
2.
Shinohara Y, Toyohira Y, Ueno S, Liu M, Tsutsui M, Yanagihara N. (2007) Effects of resveratrol, a grape polyphenol, on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. Biochemical. Pharmacol.
3.
Shinkai K, Toyohira Y, Yoshimura R,
Tsutsui M, Ueno S, Nakamura J, Yanagihara
N. (2007) Stimulation of catecholamine synthesis via activation of p44/42 MAPK in cultured bovine adrenal medullary cells by milnacipran. Naunyn-Schmiedeberg’s Archives of Pharmacology 375:65-72
4.
Takahashi K, Toyohira Y, Ueno S, Tsutsui M, Yanagihara N. (2006) Capsaicin
inhibits catecholamine secretion and synthesis by blocking Na+ and Ca2+ influx through a vaniloid receptor-independent pathway in bovine adrenal medullary cells. Naunyn-Schmiedeberg’s Archives of Pharmacology 374:107-116
5.
Uezono Y, Toyohira Y, Yanagihara N, Wada A, Taniyama K. (2006) Inhibition
by selenium compounds of catecholamine secretion due to inhibition of Ca2+ influx in cultured bovine adrenal chromaffin cells. J Pharmacol Sci 101: 223-229,
6.
Yanagihara N, Lui M, Toyohira Y, Tsutsui M, Ueno S, Shinohara Y, Takahashi
K, Tanaka K. (2006) Stimulation of catecholamine synthesis through unique estrogen receptors in bovine adrenomedullary plasma membrane by 17?-estradiol. Biochem. Biophys. Res. Commun. 339:548-553
7.
Toyohira
Y, Kubo T, Watanabe M, Uezono Y, Ueno S, Shinkai K, Tsutsui M, Izumi F, Yanagihara N. (2005) Selective blockade of nicotinic acetylcholine receptors by pimobendan, adrug for the treatment of heartfailure: reduction of catecholamine secretion and synthesis in adrenal medullary cells. Naunyn-Schmiedeberg’s Archives of Pharmacology 371:107-113
8.
Shinkai K, Yoshimura R, Toyohira Y, Ueno S, Tsutsui M, Nakamura J, Yanagihara
N. (2005) Effect of prolonged exposure to milnacipran on norepinephrine transporter in cultured bovine adrenal medullary cells. Biochemical. Pharmacol. 70:1389-1397
9.
Yoshimura R, Shinkai K, Toyohira Y, Ueno S, Yanagihara N, Nakamura J. (2005) Effect of zotepine and olanzapine on noradrenaline transporter in cultured bovine adrenal medullary cells. Hum. Psychopharmacol. Clin. Exp. 20:1-8
10. Yanagihara N, Toyohira Y, Ueno S, Tsutsui M, Utsunomiya K, Lui M, Tanaka
K. (2005) Stimulation of catecholamine synthesis by environmental estrogenic
pollutants. Endocrinorogy 146:265-272
11. Toyohira Y,
Utsunomiya K, Ueno S, Minami K, Uezono Y, Yoshimura R, Tsutsui M, Izumi F, (2003)
Yanagihara N. Inhibition of the norepinephrine transporter function in cultured bovine
adrenal medullary cells by bisphenol A. Biochemical. Pharmacol. 65: 2049-2054
12. Kawada Y, Ueno S, Asayama K, Tsutsui M, Utsunomiya K, Toyohira Y, Morisada
N, Tanaka K, Shirahata A, Yanagihara N. (2003) Stimulation of catecholamine synthesis by
orexin-a in bovine adrenal medullary cells through orexin receptor 1. Biochemical. Pharmacol. 66:141-147
13. Hara K, Minami K, Ueno S, Toyohira Y, Tsutsui M, Shigematsu A, Yanagihara N. (2002) Up-regulation of noradrenaline transporter in response to prolonged exposure to ketamine. Naunyn-Schmiedeberb’s Arch. Pharmacol. 365:406-412
14. Yoshimura R, Yanagihara N, Hara K, Nakamura J, Toyohira Y, Ueno S, Izumi
F. (2001) Dual
phases of functional change in norepinephrine transporter in cultured bovine
adrenal medullary cells by long-term treatment with clozapine. J. Neurochem. 77:1018-1026
15. Utsunomiya K, Yanagihara N, Tachikawa E, Cheah T B, Kajiwara K, Toyohira
Y, Ueno S, Izumi F. (2001) Stimulation of catecholamine synthesis in cultured bovine
adrenal medullary cells by leptin. J. Neurochem. 76:1652-1661
16. Shiraishi S, Shibuya I, Uezono Y, Yokoo H, Toyohira Y, Yamamoto R, Yanagita
T, Kobayashi H, Wada A. (2001) Heterogeneous increases of cytoplasmic calcium : Distinct effects on dowm-regulation of cell surface sodium channels and sodium channel subunit mRNA leveles. British J. pharmacol. 132:1455-1466
17. Uryu K, Minmi K, Yanagihara N, Hara K, Toyohira Y, Izumi F, Shigematsu
A. (2000) Inhibition by
neuromuscular blocking drugs of norepinephrine transporter in cultured bovine
adrenal medullary cells.. Anesth. Analg. 91: 1141-1145
18. Shibuya I, Tanaka K, Uezono Y, Ueta Y, Toyohira Y, Yanagihara N, Izumi
F, Yamashita H. (1999) Prostaglandin E2
induced Ca2+ release from ryanodin/caffeine-sensitive stores in
bovine adrenal medullary cells via EP-1-like receptors. J. Neurochem. 73:2167-2174
19. Toyohira Y, Yanagihara N, Minami K, Ueno S, Uezono Y, Tachikawa E, Kondo Y, Kashimoto T, Izumi F. (1998) Down-regulation of the noradrenaline transporter by interferon-? in cultured bovine adrenal medullary cells J. Neurochem. 70:1441-1447
20. Yoshimura R, Yanagihara N, Terao T, Uezono Y, Toyohira Y, Ueno S, Abe K,
Izumi F. (1998) Carbamazepine-induced up-regulation of voltage-dependent
Na+ channels in bovine adrenal medullary cells in culture. J. Pharmacol. Exp. Ther. 287: 441-447
21. Yanagihara N, Toyohira Y, Yamamoto H, Ohta Y, Tsutsui M, Miyamoto E, Izumi
F. (1994) Occurrence and activation of Ca2+/calmodulin-dependent protein kinase II and its endogenous substrates in bovine adrenal medullary cells. Mol. Pharmacol. 46 :423-430
22. Yanagihara N, Toyohira Y, Koda Y, Wada A, Izumi F. (1991) Inhibitory effect of okadaic acid on carbachol-evoked secretion of catecholamines in cultured bovine adrenal medullary cells. Biochem. Biophys. Res. Commun. 174:77-83
23. Izumi F, Toyohira Y, Yanagihara N, Wada A, Kobayashi H. (1986) Barium-evoked release of catecholamines from digitonin-permeabilized adrenal medullary cells. Neurosci. Lett. 69: 172-175
日本薬理学会
[文責 : 薬理学講座 更新日：2008年 1月10日]