Members

　Professor : 　Masayuki X. Mori， Ph.D.

    Lecturer   :     Ryo Okada, Ph.D.

 

Our research subject

Biomaterials, substances, and medicines are chemically processed to maintain muscular activity, brain computations, and whole body homeostasis.  Our research subject is to understand how biomaterials and medicines are processed and how such chemical processing is regulated in biological systems.  Past decades have been solved pivotal and dynamic role of ions (Na+/Ca2+) in cells in muscular and brain, however, the recent studies in the fields inspired us to research its importance to the biomaterial and chemical processing.  By building experimental system through a combination of chemical analysis equipment and bio-engineering experiment, we hope to learn how the chemical processing of bio-materials and medicines are regulated by Ca2+ signals and how its dysregulation effect on the biomaterial process and drug medications.

 

 

 

Our research expertise

Ion channel biophysics, Electrophysiology, Protein engineering, Fluorescence Energy Transfer analysis, Chemical kinetics simulation, Chemical purification (GC-, LC-chromatography), Protein crystallization and atom-level computational methods, protein-modeling, Molecular biology

 

 

 

Latest and selected publications of our research

 

Kawanabe A, Mizutani N, Polat OK, Yonezawa T, Kawai T, Mori MX, Okamura Y.

Engineering an enhanced voltage-sensing phosphatase.

J Gen Physiol. 2020 May 4;152(5):e201912491. doi: 10.1085/jgp.201912491.

 

Masamune A, Kotani H, Sörgel FL, Chen JM, Hamada S, Sakaguchi R, Masson E, Nakano E, Kakuta Y, Niihori T, Funayama R, Shirota M, Hirano T, Kawamoto T, Hosokoshi A, Kume K, Unger L, Ewers M, Laumen H, Bugert P, Mori MX, Tsvilovskyy V, Weißgerber P, Kriebs U, Fecher-Trost C, Freichel M, Diakopoulos KN, Berninger A, Lesina M, Ishii K, Itoi T, Ikeura T, Okazaki K, Kaune T, Rosendahl J, Nagasaki M, Uezono Y, Algül H, Nakayama K, Matsubara Y, Aoki Y, Férec C, Mori Y, Witt H, Shimosegawa T.

Variants That Affect Function of Calcium Channel TRPV6 Are Associated With Early-Onset Chronic Pancreatitis.

Gastroenterology. 2020 May;158(6):1626-1641.e8. doi: 10.1053/j.gastro.2020.01.005.

 

Jouraku A, Kuwazaki S, Miyamoto K, Uchiyama M, Kurokawa T, Mori E, Mori MX, Mori Y, Sonoda S.

Ryanodine receptor mutations (G4946E and I4790K) differentially responsible for diamide insecticide resistance in diamondback moth, Plutella xylostella L.

Insect Biochem Mol Biol. 2020 Mar;118:103308. doi: 10.1016/j.ibmb.2019.103308.

 

Polat OK, Uno M, Maruyama T, Tran HN, Imamura K, Wong CF, Sakaguchi R, Ariyoshi M, Itsuki K, Ichikawa J, Morii T, Shirakawa M, Inoue R, Asanuma K, Reiser J, Tochio H, Mori Y, Mori MX.

Contribution of Coiled-Coil Assembly to Ca2+/Calmodulin-Dependent Inactivation of TRPC6 Channel and its Impacts on FSGS-Associated Phenotypes.

J Am Soc Nephrol. 2019 30. 1587-1603 doi: 10.1681/ASN.2018070756.

 

Yamazaki D, Hasegawa A, Funato Y, Tran HN, Mori MX, Mori Y, Sato T, Miki H.  

Cnnm4 deficency suppresses Ca2+ signaling and promotes cell proliferation in the colon epithelia. 

Oncogene. doi: 10.1038/s41388-019-0682-0, 2019

 

Ohara-Imaizumi M, Aoyagi K, Yamauchi H, Yoshida M, Mori MX, Hida Y, Tran HN, Ohkura M, Abe M, Akimoto Y, Nakamichi Y, Nishiwaki C, Kawakami H, Hara K, Sakimura K, Nagamatsu S, Mori Y, Nakai J, Kakei M, Ohtsuka T. ELKS/Voltage-Dependent Ca2+ Channel-β Subunit Module Regulates Polarized Ca2+ Influx in Pancreatic β cells.

Cell Rep.; 26(5):1213-1226.e7, 2019

 

Tsuchiya M, Hara Y, Okuda M, Itoh K, Nishioka R, Shiomi A, Nagao K, Mori M, Mori Y, Ikenouchi J, Suzuki R, Tanaka M, Ohwada T, Aoki J, Kanagawa M, Toda T, Nagata Y, Matsuda R, Takayama Y, Tominaga M, Umeda M.

Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation.

Nat Commun. ; 9(1):2049, 2018.

 

Okada R, Zhang X, Harada Y, Wu Z, Nakanishi H:

Cathepsin H deficiency in mice induces excess Th1 cell activation and early-onset of EAE though impairment of toll-like receptor 3 cascade.

Inflamm Res ; 67(5):371-374, 2018.

 

Minobe E, Mori MX, Kameyama M:

Calmodulin and ATP support activity of the Cav1.2 channel through dynamic interactions with the channel.

J Physiol ; 595(8): 2465-77, 2017.

 

Hu Y, Duan Y, Takeuchi A, Hai-Kurahara L, Ichikawa J, Hiraishi K, Numata T, Ohara H, Iribe G, Nakaya M, Mori MX, Matsuoka S, Ma G, Inoue R:

Uncovering the arrhythmogenic potential of TRPM4 activation in atrial-derived HL-1 cells using novel recording and numerical approaches.

Cardiovasc Res ; 113(10): 1243-55, 2017.

 

Hirano M, Takada Y, Wong CF, Yamaguchi K, Kotani H, Kurokawa T, Mori MX, Snutch TP, Ronjat M, De Waard M, Mori Y:

C-terminal splice variants of P/Q-type Ca2+ channel CaV2.1 alpha1 subunits are differentially regulated by Rab3-interacting molecule proteins.

J Biol Chem ; 292(22):9365-81, 2017.

 

Sawamura S, Hatano M, Takada Y, Hino K, Kawamura T, Tanikawa J, Nakagawa H, Hase H, Nakao A, Hirano M, Rotrattanadumrong R, Kiyonaka S, Mori MX, Nishida M, Hu Y, Inoue R, Nagata R, Mori Y:

Screening of Transient Receptor Potential Canonical Channel Activators Identifies Novel Neurotrophic Piperazine Compounds.

Mol Pharmacol ; 89(3):348-63, 2016 

 

Mori MX, Itsuki K, Hase H, Sawamura S, Kurokawa T, Mori Y,and Inoue R

Dynamics of receptor-operated Ca2+ Currents Through TRPC Channels Controlled via the PI(4,5)P2-PLC Signaling Pathway

Frontiers in Pharmacol. 2015; 6: 22. doi: 10.3389/fphar.2015.00022

 

Soong TW, Mori MX:

Post-transcriptional modifications and "Calmodulation" of voltage-gated calcium channel

function: Reflections by two collaborators of David T Yue.

Channels ; 10(1):14-9, 2015.

 

Mori MX:

Bridge between the channel and FRET of PtdIns(4,5)P(2) sensor.

Channels ; 8(4):292-3, 2015.

 

Takada Y, Hirano M, Kiyonaka S, Ueda Y, Yamaguchi K, Nakahara K, Mori MX, Mori Y:

Rab3 interacting molecule 3 mutations associated with autism alter regulation of voltage-dependent Ca(2)(+) channels.

Cell Calcium ; 58(3):296-306, 2015

 

Okada R, Wu Z, Zhu A, Ni J, Zhang J, Yoshimine Y, Peters C, Saftig P, Nakanishi H:

Cathepsin D deficiency induces oxidative damage in brain pericytes and impairs the blood-brain barrier.

Mol Cell Neurosci ; 64:51-60, 2015.

 

Mori MX, Inoue R:

New experimental trends for phosphoinositides research on ion transporter/channel regulation.

J Pharmacol Sci; 126(3):186-97, 2014.

 

Takahashi N, Hamada-Nakahara S, Itoh Y, Takemura K, Shimada A, Ueda Y, Kitamata M, Matsuoka R, Hanawa-Suetsugu K, Senju Y, Mori MX, Kiyonaka S, Kohda D, Kitao A, Mori Y, Suetsugu S:

TRPV4 channel activity is modulated by direct interaction of the ankyrin domain to PI(4,5)P(2).

Nat Commun ; 5:4994, 2014

 

Itsuki K, Imai Y, Hase H, Okamura Y, Inoue R, Mori MX:

PLC-mediated PI(4,5)P-2 hydrolysis regulates activation and inactivation of TRPC6/7 channels.

Journal of General Physiology ; 143(2):183-201, 2014.

 

Zhang X, Wu Z, Hayashi Y, Okada R, Nakanishi H:

Peripheral role of cathepsin S in Th1 cell-dependent transition of nerve injury-induced acute pain to a chronic pain state.

J Neurosci ; 19;34(8):3013-3022, 2014.

 

Higurashi N, Uchida T, Lossin C, Misumi Y, Okada Y, Akamatsu W, Imaizumi Y, Zhang B, Nabeshima K, Mori MX, Katsurabayashi S, Shirasaka Y, Okano H, Hirose S:

A human Dravet syndrome model from patient induced pluripotent stem cells.

Mol Brain ;6 : 19, 2013

 

Tung TT, Nagaosa K, Fujita Y, Kita A, Mori H, Okada R, Nonaka S, Nakanishi Y:

Phosphatidylserine recognition and induction of apoptotic cell clearance by Drosophila engulfment receptor Draper.

J Biochem ; 153(5):483-491, 2013.

 

Wu Z, Zhu A, Takayama F, Okada R, Liu Y, Harada Y, Wu S, Nakanishi H:

Brazilian green propolis suppresses the hypoxia-induced neuroinflammatory responses by inhibiting NF-κB activation in microglia.

Oxid Med Cell Longev ; 2013:906726, 2013.

 

Hayashi Y, Koyanagi S, Kusunose N, Takayama F, Okada R, Wu Z, Ohdo S, Nakanishi H:

Diurnal spatial rearrangement of microglial processes through the rhythmic expression of P2Y12 receptors.

J Neurol Disord ; 1 : 2, 2013.

 

Takayama F, Wu Z, Ma HM, Okada R, Hayashi Y, Nakanishi H:

Possible involvement of aiPLA2 in the phosphatidylserine-containing liposomes induced production of PGE2 and PGD2 in microglia.

J Neuroimmunol ; 262(1-2):121-124, 2013.

 

Wu Z, Sun L, Hashioka S, Yu S, Schwab C, Okada R, Hayashi Y, McGeer PL, Nakanishi H:

Differential pathways for interleukin-1β production activated by chromogranin A and amyloid β in microglia.

Neurobiol Aging ; 34(12):2715-2725, 2013.

 

Hayashi Y, Koyanagi S, Kusunose N, Okada R, Wu Z, Tozaki-Saitoh H, Ukai K, Kohsaka S, Inoue K, Ohdo S, Nakanishi H:

The intrinsic microglial molecular clock controls synaptic strength via the circadian expression of cathepsin S.

Sci Rep ; 3:2744, 2013.

 

Itsuki K, Imai Y, Okamura Y, Abe K, Inoue R, Mori MX：

Voltage-sensing phosphatase reveals temporal regulation of TRPC3/C6/C7 channels by

membrane phosphoinositides.

Channels ; 6(3):206-9, 2012

 

Imai Y, Itsuki K, Okamura Y, Inoue R, Mori MX:

A self-limiting regulation of vasoconstrictor-activated TRPC3/C6/C7 channels coupled to PI(4,5)P(2)-diacylglycerol signalling.

J Physiol ; 590(5):1101-19, 2012.

 

Okada R, Nagaosa K, Kuraishi T, Nakayama H, Yamamoto N, Nakagawa Y, Dohmae N, Shiratsuchi A, Nakanishi Y:

Apoptosis-dependent externalization and involvement in apoptotic cell clearance of DmCaBP1, an endoplasmic reticulum protein of Drosophila.

J Biol Chem ; 287(5):3138-3146, 2012.

 

Mori MX, Imai Y, Itsuki K, Inoue R:

Quantitative measurement of Ca(2+)-dependent calmodulin-target binding by Fura-2 and CFP and YFP FRET imaging in living cells.

Biochemistry ; 50(21):4685-96, 2011.

 

Nagaosa K, Okada R, Nonaka S, Takeuchi K, Fujita Y, Miyasaka T, Manaka J, Ando I, Nakanishi Y:

Integrin βν-mediated phagocytosis of apoptotic cells in Drosophila embryos.

J Biol Chem ; 286(29):25770-25777, 2011.

 

Inoue R, Jensen LJ, Jian Z, Shi J, Hai L, Lurie AI, Henriksen FH, Salomonsson M, Morita H, Kawarabayashi Y, Mori M, Mori Y, Ito Y.:

Synergistic Activation of Vascular TRPC6 Channel by Receptor and Mechanical Stimulation via Phospholipase C/Diacylglycerol and Phospholipase A(2)/omega-Hydroxylase/20-HETE Pathways.

Circulation Research ; 104(12):1399-409, 2009.

 

Mori MX, Vander Kooi CW, Leahy DJ, Yue DT:

Crystal structure of the CaV2 IQ domain in complex with Ca2+/calmodulin: high-resolution mechanistic implications for channel regulation by Ca2+.

Structure ; 16(4):607-20, 2008.

 

Yang PS, Alseikhan BA, Hiel H, Grant L, Mori MX, Yang W, Fuchs PA, Yue DT:

Switching of Ca2+-dependent inactivation of Ca(v)1.3 channels by calcium binding proteins of auditory hair cells.

J Neurosci ; 26(42):10677-89, 2006.

 

Wada T, Imanishi T, Kawaguchi A, Mori MX, Mori Y, Imoto K, Ichida S:

Effects of calmodulin and Ca2+ channel blockers on omega-conotoxin GVIA binding to crude membranes from alpha1B subunit (Cav2.2) expressed BHK cells and mice brain lacking the alpha1B subunits.

Neurochem Res; 30(8):1045-54, 2005.

 

Mori MX, Erickson MG, Yue DT. Functional stoichiometry and local enrichment of calmodulin interacting with Ca2+ channels.

Science ; 304(5669):432-5, 2004.

 

Shi J, Mori E, Mori Y, Mori M, Li J, Ito Y, Inoue R:

Multiple regulation by calcium of murine homologues of transient receptor potential proteins TRPC6 and TRPC7 expressed in HEK293 cells.

Journal of Physiology ; 561(2):415-32, 2004.

 

Weiss LA, Escayg A, Kearney JA, Trudeau M, MacDonald BT, Mori M, Reichert J, Buxbaum JD, Meisler MH:

Sodium channels SCN1A, SCN2A and SCN3A in familial autism.

Molecular Psychiatry; 8(2):186-94,2003.

 

Liang HY, DeMaria CD, Erickson MG, Mori MX, Alseikhan BA, Yue DT:

Unified mechanisms of Ca2+ regulation across the Ca2+ channel family.

Neuron ; 39(6):951-60, 2003.

 

Erickson MG, Liang HY, Mori MX, Yue DT:

FRET two-hybrid mapping reveals function and location of L-type Ca2+ channel CaM preassociation.

Neuron ; 39(1):97-107, 2003.

 

Akiba I, Seki T, Mori M, Iizuka M, Nishimura S, Sasaki S, Imoto K, Barsoumian EL:

Stable expression and characterization of human PN1 and PN3 sodium channels.

Receptors & Channels ; 9(5):291-9,2 003.

 

Mori M, Konno T, Ozawa T, Murata M, Imoto K, Nagayama K:

Novel interaction of the voltage-dependent sodium channel (VDSC) with calmodulin

Biochemistry ; 39(6):1316-23, 2000.

 

 

　

Our class in Medical school

Biomaterials and chemistry; this class is focusing to learn the physico-chemical backgrounds of materials (substances) in human body and medicine.

 

 

For researchers and students

If you have an interest to collaborate or work in here, we are very well-comes.  Contact

address: j-stibst@mbox.med.uoeh-u.ac.jp